JoVE Journal

Bioengineering

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Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

This article contains a set of protocols for the development of human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) networks cultured on multiwell MEA plates to reversibly electroporate the cell membrane for action potential measurements. High-throughput recordings are obtained from the same cell sites repeatedly over days.

Rozdziały w tym wideo

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Title

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Pre-plating of the Cryopreserved hiPSC-CM for Maturation

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Multiwell MEA Plate Sterilization and Coating

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hiPSC-CM Dissociation and Plating on Multiwell MEA Plate

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hiPSC-CM Electroporation and Signal Acquisition

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Data Segmentation and Analysis

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Results: Action Potential Recordings of hiPSC-CMs

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Conclusion

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