JoVE Logo

Zaloguj się

Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization

7.8K Views

08:06 min

January 17th, 2020

DOI :

10.3791/60135-v

January 17th, 2020


Przeglądaj więcej filmów

Ventricular like Cardiomyocytes

Rozdziały w tym wideo

0:04

Title

0:38

Induced Pluripotent Stem Cell (iPSC) Passaging and Maintenance

1:40

Human Embryonic Stem Cell (hESC)-Qualified Matrix-Coated Plates and Coverslips

2:14

Selected iPSC-Cardiomyocyte (CM) Plating

3:39

Intracellular Calcium (Ca2+) Transient Assessment

5:42

Results: Representative iPSC-CM Phenotypic Characterization, Differentiation Efficiency, and Calcium Amplitude Analyses

7:16

Conclusion

Powiązane Filmy

article

08:37

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

6.9K Views

article

14:39

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

17.2K Views

article

05:38

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

4.0K Views

article

08:53

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

11.5K Views

article

09:23

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

3.5K Views

article

14:03

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

1.8K Views

article

08:29

A Cardiac Microphysiological System for Studying Ca2+ Propagation via Non-genetic Optical Stimulation

471 Views

article

10:01

High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening

3.0K Views

article

10:30

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

2.8K Views

article

06:59

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

7.1K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone