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Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization

7.9K Views

08:06 min

January 17th, 2020

DOI :

10.3791/60135-v

January 17th, 2020


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Ventricular like Cardiomyocytes

Chapters in this video

0:04

Title

0:38

Induced Pluripotent Stem Cell (iPSC) Passaging and Maintenance

1:40

Human Embryonic Stem Cell (hESC)-Qualified Matrix-Coated Plates and Coverslips

2:14

Selected iPSC-Cardiomyocyte (CM) Plating

3:39

Intracellular Calcium (Ca2+) Transient Assessment

5:42

Results: Representative iPSC-CM Phenotypic Characterization, Differentiation Efficiency, and Calcium Amplitude Analyses

7:16

Conclusion

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