Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA

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22.0K Views

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06:46 min

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November 9th, 2019

DOI :

10.3791/60143-v

November 9th, 2019

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Marc Herb¹, Alina Farid¹, Alexander Gluschko¹, Martin Krönke¹^,²^,³^,⁴, Michael Schramm¹

¹Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, ²Center for Molecular Medicine Cologne, ³Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), ⁴German Center for Infection Research (DZIF)

Podsumowanie

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RNA Transcription Kit

Microvolume Spectrophotometer

Denaturing Agarose Gel

Rozdziały w tym wideo

0:04

Title

0:43

mRNA Generation and Purification

2:59

Transfection of Macrophages with mRNA

4:27

Results: Highly Efficient Transfection of Peritoneal Macrophages and Bone Marrow-Derived Macrophages with in vitro Transcribed mRNA

6:14

Conclusion

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