Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

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8.7K Views

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10:38 min

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November 22nd, 2019

DOI :

10.3791/60223-v

November 22nd, 2019

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Preston D. Crowell*¹, Jenna M. Giafaglione*¹, Takao Hashimoto², Johnny A. Diaz², Andrew S. Goldstein²^,³^,⁴^,⁵^,⁶

¹Molecular Biology Interdepartmental Program, University of California, Los Angeles, ²Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, ³Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, ⁴Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, ⁵Jonsson Comprehensive Cancer Center, University of California, Los Angeles, ⁶Molecular Biology Institute, University of California, Los Angeles

Podsumowanie

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Mouse Prostate Epithelial Cells

Rozdziały w tym wideo

0:05

Title

0:52

Plating Sorted Prostate Epithelial Cells into Primary Mouse Organoid Culture

3:29

Extracting Protein Lysate from Prostate Organoids for Western Blot Analysis

4:50

Fixing and Staining Prostate Organoids for Immunohistochemical Analysis by Whole-Mount Confocal Microscopy

6:44

Tissue Clearing and Mounting of the Stained Prostate Organoids for Whole-mount Confocal Microscopy

7:57

Results: Analysis of Lineage Marker Expression in Prostate Organoids by Western Blot and Whole-Mount Confocal Microscopy

9:44

Conclusion

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