Handling and Assessment of Human Primary Prostate Organoid Culture

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17.0K Views

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08:45 min

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January 17th, 2019

DOI :

10.3791/59051-v

January 17th, 2019

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Tara McCray*¹, Zachary Richards*¹, Joseph Marsili¹, Gail S. Prins¹^,²^,³, Larisa Nonn¹^,³

¹Department of Pathology, University of Illinois at Chicago, ²Departments of Urology, Physiology, and Biophysics, University of Illinois at Chicago, ³University of Illinois Cancer Center

Podsumowanie

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Tissue Culture Methods

Rozdziały w tym wideo

0:04

Title

1:04

Collection of Organoids from Matrix Gel

2:09

Formalin Fixation and Paraffin Embedding of Organoids for Histological Endpoints

4:00

Sectioning the Formalin Fixed Paraffin Embedded (FFPE) Histology Gel Blocks

5:04

Whole-mounting of Organoids for Immunofluorescent Staining

6:35

Results: Analysis of Human Primary Prostate Organoids

7:56

Conclusion

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