Methods Article

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

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8.6K Views

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09:32 min

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February 27th, 2020

DOI :

10.3791/60147-v

February 27th, 2020

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Mulu Geletu*¹^,², Victoria Hoskin*¹, Blerta Starova*¹^,³, Maximilian Niit¹^,⁴, Hanad Adan¹, Bruce Elliott¹, Patrick Gunning², Leda Raptis¹

¹Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine, Queen's University, Kingston, ²Department of Chemical and Physical Sciences, University of Toronto, Mississauga, ³Department of Laboratory Medicine, Brampton Civic Hospital Campus, William Osler Health System, ⁴Latner Thoracic Surgery Research Laboratories

Podsumowanie

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HC11 Cells

EpH4 Cells

Mouse Breast Epithelial Cells

Adipocytes Differentiation

Myotubes Differentiation

Cell Plating Technique

Trypsinization

Phase Contrast Microscopy

Rozdziały w tym wideo

0:05

Introduction

1:24

Plating and Differentiation Induction of HC11 Cells

3:36

3D Growth and Differentiation Induction of EpH4 Cells in EHS Matrix

6:24

Western Blotting for β-Casein

7:42

Results: Assessment of HC11 and EpH4 Cell Differentiation

8:49

Conclusion

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