Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Please note that all translations are automatically generated. Click here for the English version.

31.2K Views

•

10:40 min

•

October 27th, 2019

DOI :

10.3791/60510-v

October 27th, 2019

•

Fernando González Ibanez¹^,², Katherine Picard¹^,², Maude Bordeleau¹^,³, Kaushik Sharma¹^,²^,⁴, Kanchan Bisht¹^,²^,⁴, Marie-Ève Tremblay¹^,²

¹Axe Neurosciences, Centre de Recherche du CHU de Québec-Université Laval, ²Département de Médecine Moléculaire, Faculté de Médecine, Université Laval, ³Integrated Program in Neuroscience, McGill University, ⁴Center for Brain Immunology and Glia (BIG), University of Virginia

Podsumowanie

Przeglądaj więcej filmów

Immunofluorescence Staining

IBA1

TMEM119

Microglial Density

Microglial Morphology

Macrophage Infiltration

Mouse Brain Analysis

Quantitative Analysis

Anti IBA1 Antibodies

Anti TMEM119 Antibodies

Fiji

ImageJ

Image Processing Program

Nearest Neighbor Distance

Area Measurement

Channel Tool

Rozdziały w tym wideo

0:04

Title

1:00

Density and Distribution Analysis

5:50

Morphology Analysis

9:01

Results: Immunofluorescence Staining Using IBA1 and TMEM119

10:06

Conclusion

Powiązane Filmy

09:07

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

19.2K Views

09:42

Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining

21.1K Views

05:58

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

18.1K Views

12:14

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

34.8K Views

12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

15.7K Views

08:46

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

17.1K Views

07:19

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

9.5K Views

08:14

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

12.3K Views

07:03

Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration

66.3K Views

09:05

Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

9.0K Views