Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
Please note that all translations are automatically generated. Click here for the English version.
6.4K Views
•
11:17 min
•
March 10th, 2021
DOI :
March 10th, 2021
•Di Zhang*1,2, Kefang Liu*3,4, Dan Lu*2,5, Pengyan Wang1,2, Qingxu Zhang1,2, Peipei Liu2, Yingze Zhao2, Yan Chai4, Jianxin Lyu1, Jianxun Qi4, William J. Liu1,2
1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences, University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 5Savaid Medical School, University of Chinese Academy of Sciences
Przeglądaj więcej filmów
Rozdziały w tym wideo
0:04
Introduction
0:54
E. coli Transformation
1:46
Culture Inoculation
2:59
Bacteria Harvest
3:28
Inclusion Body Purification
4:27
MHC Complex Refolding
4:53
MHC H Chain and Beta2m Injection and Dilution
5:42
Refolded Protein Concentration Determination
6:51
Crystallization, Data Collection, and Processing
7:41
Structure Determination and Analyses
8:33
Results: Representative Bat MHC I Beta2-Microglobulin Stability and Structure Analysis
10:21
Conclusion
Powiązane Filmy
19:05
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
12.3K Views
08:03
Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
2.0K Views
09:32
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
11.7K Views
09:53
Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens
11.4K Views
08:04
Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
6.9K Views
10:01
Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice
8.0K Views
12:09
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
9.7K Views
13:51
The MultiBac Protein Complex Production Platform at the EMBL
15.9K Views
08:07
Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry
3.4K Views
07:59
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
14.9K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone