Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
Please note that all translations are automatically generated. Click here for the English version.
6.3K Views
•
08:04 min
•
November 19th, 2020
DOI :
November 19th, 2020
•1Center for Genomic Medicine, Massachusetts General Hospital, 2Department of Psychiatry, Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, 4Department of Psychiatry, Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program, McLean Hospital
Rozdziały w tym wideo
0:04
Introduction
0:38
Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
1:46
iPSC-Derived BMEC Subculture and Purification
3:02
Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
4:09
Efflux Transporter Activity and Analysis
5:22
Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
7:18
Conclusion
Powiązane Filmy
09:36
Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
18.8K Views
06:36
An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
6.0K Views
12:02
Isolation and Culture of Endothelial Cells from the Embryonic Forebrain
15.4K Views
10:20
Generation of a Human iPSC-Based Blood-Brain Barrier Chip
12.3K Views
04:23
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
2.1K Views
10:34
Derivation of a Human Brain Organoid with Microglia Development
705 Views
10:12
Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells
2.3K Views
09:03
Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
2.0K Views
04:41
Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells
1.5K Views
01:34
Generowanie komórek śródbłonka mózgu z indukowanych pluripotencjalnych komórek macierzystych
32 Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone