Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

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6.3K Views

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08:04 min

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November 19th, 2020

DOI :

10.3791/61629-v

November 19th, 2020

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Sovannarath Pong¹^,²^,³, Paulo Lizano¹^,²^,³^,⁴, Rakesh Karmacharya¹^,³^,⁴^,⁵

¹Center for Genomic Medicine, Massachusetts General Hospital, ²Department of Psychiatry, Beth Israel Deaconess Medical Center, ³Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, ⁴Department of Psychiatry, Harvard Medical School, ⁵Schizophrenia and Bipolar Disorder Program, McLean Hospital

Podsumowanie

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Brain Microvascular Endothelial Cells

Human Induced Pluripotent Stem Cells

Disease specific BMECs

Supernatant Replacement

Efflux Transporter Activity

Rozdziały w tym wideo

0:04

Introduction

0:38

Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)

1:46

iPSC-Derived BMEC Subculture and Purification

3:02

Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis

4:09

Efflux Transporter Activity and Analysis

5:22

Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation

7:18

Conclusion

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