Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

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2.1K Views

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04:23 min

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March 31st, 2021

DOI :

10.3791/62391-v

March 31st, 2021

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Elizabeth A. Nelson¹^,², Jingyao Qiu³^,⁴, Nicholas W. Chavkin¹^,², Karen K. Hirschi¹^,²^,³^,⁴^,⁵

¹Department of Cell Biology, University of Virginia, ²Cardiovascular Research Center, University of Virginia, ³Department of Medicine, Yale University School of Medicine, ⁴Department of Genetics, Yale University School of Medicine, ⁵Yale Cardiovascular Research Center, Yale University School of Medicine

Podsumowanie

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Hemogenic Endothelial Cells

Human Pluripotent Stem Cells

Endothelial to hematopoietic Transition

Embryonic Stem Cells

Differentiation Protocol

FACS Sorting

CD45 negative Cells

CD31 positive Cells

VE cadherin negative Cells

C Kit positive Cells

CD34 positive Cells

KDR positive Cells

Colony Forming Unit Erythroid

Colony Forming Unit Granulocyte Macrophage

Hematopoietic Progenitor Cells

Rozdziały w tym wideo

0:04

Introduction

0:25

Differentiation of Human Embryonic Stem Cells to Hemogenic Endothelial Cells

1:17

FACS-Isolation of Hemogenic Endothelial Cells

2:56

Results: Hematopoietic Potential of Hemogenic Endothelial Cells

3:54

Conclusion

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