Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
6.3K Views
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08:04 min
•
November 19th, 2020
DOI :
November 19th, 2020
•1Center for Genomic Medicine, Massachusetts General Hospital, 2Department of Psychiatry, Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, 4Department of Psychiatry, Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program, McLean Hospital
Chapters in this video
0:04
Introduction
0:38
Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
1:46
iPSC-Derived BMEC Subculture and Purification
3:02
Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
4:09
Efflux Transporter Activity and Analysis
5:22
Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
7:18
Conclusion
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