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Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

6.3K Views

08:04 min

November 19th, 2020

DOI :

10.3791/61629-v

November 19th, 2020


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Brain Microvascular Endothelial Cells

Chapters in this video

0:04

Introduction

0:38

Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)

1:46

iPSC-Derived BMEC Subculture and Purification

3:02

Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis

4:09

Efflux Transporter Activity and Analysis

5:22

Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation

7:18

Conclusion

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