Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

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2.9K Views

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09:09 min

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February 24th, 2021

DOI :

10.3791/61780-v

February 24th, 2021

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Alexander C. Y. Foo¹, Peter M. Thompson¹, Geoffrey A. Mueller¹

¹Nuclear Magnetic Resonance Group, National Institute of Environmental Health Sciences

Podsumowanie

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Molecular Weight Cutoff

Fluorescence Absorbance

Lyophilization

Refolding Buffer

Rozdziały w tym wideo

0:04

Introduciton

1:13

Endogenous Lipid Removal

2:42

Reconstitution of Apo- and Cargo-loaded Bla g 1

5:07

Phospholipid Cargo Removal/Loading Confirmation

6:22

Results: Representative Bla g 1 Purification, Lipid Removal Verification, and Recovery

8:18

Conclusion

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