Results: Representative Bla g 1 Purification, Lipid Removal Verification, and Recovery
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Conclusion
Transcription
Many allergens bind hydrophobic molecules. This protocol enables the complete removal and replacement of these ligands, allowing us to study their impact on structure and immunogenicity in a systematic manner. The use of reverse-phase HPLC, couple
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This protocol describes the removal of endogenous lipids from allergens, and their replacement with user-specified ligands through reverse-phase HPLC coupled with thermal annealing. 31P-NMR and circular dichroism allow for the rapid confirmation of ligand removal/loading, and the recovery of native allergen structure.