Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

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7.3K Views

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09:21 min

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March 26th, 2021

DOI :

10.3791/62045-v

March 26th, 2021

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Justin A. Courson¹, Paul T. Landry¹, Thao Do¹, Eric Spehlmann², Pascal J. Lafontant², Nimesh Patel¹, Rolando E. Rumbaut³^,⁴, Alan R. Burns¹^,⁴

¹College of Optometry, University of Houston, ²Department of Biology, DePauw University, ³Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey Veterans Affairs Medical Center, ⁴Children’s Nutrition Center, Baylor College of Medicine

Podsumowanie

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Serial Block Face Scanning Electron Microscopy

SBF SEM

Biological Tissue Samples

Three dimensional Datasets

Electron Microscopy

Tissue Ultra Structures

Imaging Technique

Cellular Ultra Structure

Staining Protocol

Osmium Tetroxide

Uranyl Acetate

Lead Aspartate Solution

Tissue Dehydration

Resin Infiltration

Acetone Series

Rozdziały w tym wideo

0:04

Introduction

1:05

Tissue Processing

4:19

Block Preparation

6:59

Results: Representative Serial Block-Face Scanning Electron Microscopy (SBF-SEM) Imaging

8:25

Conclusion

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