Results: Representative Serial Block-Face Scanning Electron Microscopy (SBF-SEM) Imaging
8:25
Conclusion
Transkript
Serial block-face scanning electron microscopy provides an unprecedented view of three-dimensional tissue ultra structures, and can answer a host of questions previously impossible or exceedingly difficult to pursue. This technique allows the rapi
Melden Sie sich an oder starten Sie Ihre kostenlose Testversion, um auf diese Inhalt zuzugreifen
This protocol outlines a routine method for using serial block-face scanning electron microscopy (SBF-SEM), a powerful 3D imaging technique. Successful application of SBF-SEM hinges on proper fixation and tissue staining techniques, as well as careful consideration of imaging settings. This protocol contains practical considerations for the entirety of this process.