Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
Please note that all translations are automatically generated. Click here for the English version.
4.1K Views
•
08:43 min
•
May 7th, 2021
DOI :
May 7th, 2021
•Ruibing Xia1,2,3, Simone Loy1, Stefan Kääb1,3, Anna Titova1, Christian Schulz1,2,3, Steffen Massberg1,2,3, Sebastian Clauss1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)
Rozdziały w tym wideo
0:05
Introduction
0:58
Microdissection of SAN and AVN
3:14
Digestion
4:02
Magnetic Enrichment of CD45 and Sample Staining
5:28
Cell Sorting and Gating Strategy
6:10
Results: Identification of SAN and AVN and Analysis of Sorted Cardiac Macrophages
8:17
Conclusion
Powiązane Filmy
17:42
A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
63.4K Views
07:26
Isolation of Functional Cardiac Immune Cells
15.0K Views
08:24
Isolation and Identification of Extravascular Immune Cells of the Heart
12.5K Views
07:25
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
7.9K Views
08:34
Isolation of Atrial Myocytes from Adult Mice
10.3K Views
06:27
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
5.2K Views
09:32
Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
13.1K Views
11:53
Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart
7.2K Views
06:22
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
3.0K Views
02:55
Izolacja węzła zatokowo-przedsionkowego z modelu mysiego: procedura zbierania węzła SA z mysiego serca
1.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone