Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades")
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4.8K Views
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09:23 min
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March 31st, 2021
DOI :
March 31st, 2021
•1LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, 2CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France
Rozdziały w tym wideo
0:05
Introduction
0:56
Nanoblade Preparation
2:45
Concentration of Nanoblades on a Sucrose-Cushion
4:16
Monitoring Cas9 Loading Within Nanoblades and Transduction of Cells
6:24
Results: Characterization of Nanoblades
8:29
Conclusion
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