Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging

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9.7K Views

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11:57 min

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February 24th, 2021

DOI :

10.3791/62267-v

February 24th, 2021

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Adrien Bertolo*¹, Mohamed Nouhoum*¹, Silvia Cazzanelli*¹, Jeremy Ferrier*³, Jean-Charles Mariani², Andrea Kliewer⁴^,², Benoit Belliard¹, Bruno-Félix Osmanski³, Thomas Deffieux*¹, Sophie Pezet*¹, Zsolt Lenkei*², Mickael Tanter*¹

¹Physics for Medicine Paris, ESPCI Paris, INSERM, CNRS, PSL Research University, ²Institute of Psychiatry and Neurosciences of Paris, INSERM U1266, Université de Paris, ³Iconeus, ⁴Department of Pharmacology and Toxicology, Jena University Hospital - Friedrich Schiller University Jena

Podsumowanie

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Whole Brain 3D Activation

Functional Connectivity Mapping

Transcranial Functional Ultrasound Imaging

Neuroimaging Modality

Cerebral Blood Volume

Ultra Fast Plane Wave Imaging

Hemodynamic Responses

Anesthetized Mice

Awake Animal Experiments

Functional Ultrasound

FMRI Comparison

Behavioral Studies

Electrocardiogram Recording

Stereotactic Frame

Surgical Procedures

Head Fixation

Rozdziały w tym wideo

0:05

Introduction

1:22

Animal Preparation

3:58

Probe Positioning

4:34

Angiographic Scan and Atlas Registration

5:49

Brain Positioning System (BPS)

7:14

Task-Evoked Experiment: Whisker Stimulation

8:14

4D Functional Connectivity

9:08

Results: Transcranial Activation Maps and rCBV Time Course Following Whiskers Stimulation in an Anesthetized and Awake Behaving Mouse

11:08

Conclusion

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