Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

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07:00 min

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August 5th, 2021

DOI :

10.3791/62350-v

August 5th, 2021

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Claudine Bisson¹^,², Corey W. Hecksel³^,⁴, James B. Gilchrist³, M. Alejandra Carbajal¹, Roland A. Fleck¹

¹Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, ²Department of Biological Science, Birkbeck College, University of London, ³Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, ⁴SLAC National Accelerator Laboratory, Stanford University

Podsumowanie

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Cryo electron Tomography

Vitreous Lamellae

Plasmodium Falciparum

Red Blood Cells

Dual Beam Scanning Electron Microscope

Cryo FIB SEM

High Resolution Imaging

Rozdziały w tym wideo

0:04

Introduction

0:48

Screening Plunge Frozen Grids and Preparing for FIB-Milling

1:28

On-Grid FIB Milling of Plunge Frozen Cells

4:18

Results: Analysis of Cryo-ET Data from FIB-Milled Lamella

6:28

Conclusion

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