Slicing of the Agarose-Embedded Tissue Using a Vibratome
3:51
Culture of Organotypic Slice Cultures
4:38
Formalin Fixation and Paraffin Embedding of OTSCs
6:20
Hematoxylin and Eosin Staining
7:09
Results: Morphology, Viability, and Histopathological Evaluation of the OTSCs at 6 and 15 Days of Primary PDAC and Metastasis
8:50
Conclusion
Transkript
Organotypic tissue slices have been generated from various tissues. Due to the preserved tissue integrity and the development of cultivation techniques, they have been suitable for modeling complex physiological mechanisms. Organotypic slice cultu
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This protocol describes the preparation of organotypic slice cultures (OTSCs). This technique facilitates the ex vivo cultivation of intact multicellular tissue. OTSCs can be used immediately to test for their respective response to drugs in a multicellular environment.