Organotypic Slice Cultures as Preclinical Models of Tumor Microenvironment in Primary Pancreatic Cancer and Metastasis
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2.4K Views
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09:19 min
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June 22nd, 2021
DOI :
June 22nd, 2021
•Rüdiger Braun1, Olha Lapshyna1, Susanne Eckelmann2,3, Kim Honselmann1, Louisa Bolm1, Meike ten Winkel1, Steffen Deichmann1, Oliver Schilling4, Charli Kruse2,3, Tobias Keck1, Ulrich Wellner1, Peter Bronsert4,5,6, Matthias Brandenburger3
1Department of Surgery, University Medical Center Schleswig-Holstein, 2Institute of Medical and Marine Biotechnology, University of Lübeck, 3Fraunhofer Research and Development Center for Marine and Cellular Biotechnology, 4Institute for Surgical Pathology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 5Tumorbank Comprehensive Cancer Center Freiburg, Medical Center - University of Freiburg, 6Core Facility Histopathology and Digital Pathology Freiburg, Medical Center - University of Freiburg
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Rozdziały w tym wideo
0:00
Introduction
0:54
Preparation of Low-Melting Agarose and Vibratome
1:58
Tissue Embedding in Low-Melting Agarose
3:13
Slicing of the Agarose-Embedded Tissue Using a Vibratome
3:51
Culture of Organotypic Slice Cultures
4:38
Formalin Fixation and Paraffin Embedding of OTSCs
6:20
Hematoxylin and Eosin Staining
7:09
Results: Morphology, Viability, and Histopathological Evaluation of the OTSCs at 6 and 15 Days of Primary PDAC and Metastasis
8:50
Conclusion
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