Methods Article

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

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6.1K Views

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07:59 min

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September 3rd, 2021

DOI :

10.3791/62630-v

September 3rd, 2021

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Hiroki Kobayashi¹^,²^,³^,⁴, Krystyna A. Gieniec¹^,², Jia Q. Ng¹^,², Jarrad Goyne¹^,², Tamsin R. M. Lannagan¹^,², Elaine M. Thomas¹^,², Georgette Radford¹^,², Tongtong Wang¹^,², Nobumi Suzuki¹^,²^,⁵, Mari Ichinose¹^,², Josephine A. Wright², Laura Vrbanac¹^,², Alastair D. Burt⁶, Masahide Takahashi³^,⁴^,⁷, Atsushi Enomoto³, Daniel L. Worthley², Susan L. Woods¹^,²

¹Adelaide Medical School, University of Adelaide, ²South Australian Health and Medical Research Institute (SAHMRI), ³Department of Pathology, Nagoya University Graduate School of Medicine, ⁴Division of Molecular Pathology, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, ⁵Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, ⁶Translational and Clinical Research Institute, Newcastle University, ⁷International Center for Cell and Gene Therapy, Fujita Health University

Podsumowanie

Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.

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Colorectal Cancer

Liver Metastasis

Portal Vein Injection

Tumor Microenvironment

CAS cancer associated Stroma

Therapeutic Strategies

Tumoroids

Surgical Procedure

Pain Management

Aseptic Surgical Area

Buprenorphine