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Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

2.1K Views

12:29 min

March 11th, 2022

DOI :

10.3791/63231-v

March 11th, 2022


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Null Mutants

Rozdziały w tym wideo

0:04

Introduction

1:01

Construction of pDM4 Recombinant Plasmid with PCR In-Frame Deletion Products

3:10

Preparation of Electrocompetent E. coli and Electroporation of pDM4 Recombinant Plasmid

4:52

Transfer of In-Frame Deletion to the Aeromonas Strain

7:44

Motility Assays in Liquid and Semi-Solid Media

8:34

Isolation and Purification of Flagella

10:23

Results: Confirmation of Null Mutants and Phenotypic Analysis of Polar Flagella

11:47

Conclusion

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