Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
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12:29 min
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March 11th, 2022
DOI :
March 11th, 2022
•Rozdziały w tym wideo
0:04
Introduction
1:01
Construction of pDM4 Recombinant Plasmid with PCR In-Frame Deletion Products
3:10
Preparation of Electrocompetent E. coli and Electroporation of pDM4 Recombinant Plasmid
4:52
Transfer of In-Frame Deletion to the Aeromonas Strain
7:44
Motility Assays in Liquid and Semi-Solid Media
8:34
Isolation and Purification of Flagella
10:23
Results: Confirmation of Null Mutants and Phenotypic Analysis of Polar Flagella
11:47
Conclusion
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