Construction of pDM4 Recombinant Plasmid with PCR In-Frame Deletion Products
3:10
Preparation of Electrocompetent E. coli and Electroporation of pDM4 Recombinant Plasmid
4:52
Transfer of In-Frame Deletion to the Aeromonas Strain
7:44
Motility Assays in Liquid and Semi-Solid Media
8:34
Isolation and Purification of Flagella
10:23
Results: Confirmation of Null Mutants and Phenotypic Analysis of Polar Flagella
11:47
Conclusion
필기록
So neurminic acid derivative are only present in pathogenic bacteria, aminiglycosylated flagella contain this sugar. In frame deletion of region contained in the genes can help to find flagella glycosylation clusters. This technique, the latest sp
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Here, we describe the construction of null mutants of Aeromonas in specific glycosyltransferases or regions containing glycosyltransferases, the motility assays, and flagella purification performed to establish the involvement and function of their encoded enzymes in the biosynthesis of a glycan, as well as the role of this glycan in bacterial pathogenesis.