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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

5.0K Views

09:49 min

March 16th, 2022

DOI :

10.3791/63519-v

March 16th, 2022


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Cryo EM

Rozdziały w tym wideo

0:04

Introduction

1:27

Grid Loading, Mapping, and Selection of Best Cryo-EM Grids for Data Collection

2:36

Setting up a Data Collection Session in the Single Particle Analysis Software

5:44

Final Microscope Alignments Before Starting Data Collection

7:15

Results: Gallery of Grids, Cryo-EM Reconstruction of Apo-Ferritin, and Effect of Energy Filtering on Cryo-EM Images

8:56

Conclusion

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