Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
Please note that all translations are automatically generated. Click here for the English version.
4.9K Views
•
09:49 min
•
March 16th, 2022
DOI :
March 16th, 2022
•Rozdziały w tym wideo
0:04
Introduction
1:27
Grid Loading, Mapping, and Selection of Best Cryo-EM Grids for Data Collection
2:36
Setting up a Data Collection Session in the Single Particle Analysis Software
5:44
Final Microscope Alignments Before Starting Data Collection
7:15
Results: Gallery of Grids, Cryo-EM Reconstruction of Apo-Ferritin, and Effect of Energy Filtering on Cryo-EM Images
8:56
Conclusion
Powiązane Filmy
10:49
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
13.1K Views
13:28
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
50.2K Views
09:33
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
7.1K Views
09:06
Cryo-EM and Single-Particle Analysis with Scipion
3.7K Views
06:10
Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
4.3K Views
07:00
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
3.2K Views
05:05
Preparation of High-Temperature Sample Grids for Cryo-EM
3.5K Views
09:53
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
6.4K Views
08:32
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
3.9K Views
13:43
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
12.7K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone