JoVE Journal

Cancer Research

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CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

Please note that all translations are AI generated. Click here for the English version.

2.5K Views

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12:14 min

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June 2nd, 2022

DOI :

10.3791/64025-v

June 2nd, 2022

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Tami Green¹, Lina Šlekienė¹^,², Lena Gunhaga¹

¹Umeå Centre for Molecular Medicine, Umeå University, ²Department of Histology and Embryology, Medical Academy, Lithuanian University of Health Sciences

Podsumowanie

The CAM-Delam assay to evaluate the metastatic capacity of cancer cells is relatively fast, easy, and cheap. The method can be used for unraveling the molecular mechanisms regulating metastasis formation and for drug screening. An optimized assay for analyzing human tumor samples could be a clinical method for personalized cancer treatment.

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CAM Delam Assay

Metastatic Properties

Invasion Capacity

Metastatic Aggressiveness

Collagen RPMI Mix

Embryo Assessment

Rozdziały w tym wideo

0:05

Introduction

1:00

Egg Incubation, Preparation of Weighing Boat, Plastic Boxes, and Dissection Instruments

1:46

Opening the Eggs, Transferring to the Internal Humidified Chamber, and Preparing Silicone Rings

3:07

Preparation and Seeding the Cancer Cells on the CAM

5:31

Isolation of the CAM with Associated Cancer Cells and Sectioning CAM-Delam Samples

7:28

Immunohistochemistry Staining, Microscopy Imaging, and Delamination Scoring

9:40

Results: CAM-Delam Assay to Evaluate the Metastatic Capacity of Cancer Cells

11:33

Conclusion

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