Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

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944 Views

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09:59 min

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March 31st, 2023

DOI :

10.3791/64713-v

March 31st, 2023

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Michael C. Lee*¹^,³^,⁴, Sarah H. Gardner*²^,³^,⁴, Rodrigo Tapia Hernandez¹^,³^,⁴, Jefferson Chan¹^,²^,³^,⁴

¹Department of Chemistry, University of Illinois Urbana-Champaign, ²Department of Biochemistry, University of Illinois Urbana-Champaign, ³Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, ⁴Cancer Center of Illinois, University of Illinois Urbana-Champaign

Podsumowanie

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Aldehyde Dehydrogenase 1A1

ALDH1A1 Activity

Ovarian Cancer Cells

AlDeSense Application

Confocal Microscope Imaging

Image Processing Software

Fluorescent Cells

Cell Culture Protocol

Cell Detachment

Automated Cell Counter

Rozdziały w tym wideo

0:04

Introduction

0:38

Fluorescence Microscopy Imaging of Cells with High ALDH1A1 Activity

3:03

Application of Flow Cytometry to Identify Cells with High ALDH1A1 Activity

7:20

Results: Total ALDH1A1 Activity and Identifying the Population of ALDH1A1+ Cells

9:33

Conclusion

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