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Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

2.2K Views

09:52 min

January 6th, 2023

DOI :

10.3791/64849-v

January 6th, 2023


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CRISPR Activation System

Rozdziały w tym wideo

0:05

Introduction

0:53

Molecular Cloning

4:34

In Vivo Injection of Adeno-Associated Virus (AAV) into the Inguinal White Adipose Tissue (iWAT)

6:14

In Vitro Differentiation of Stromal Vascular Cells (SVFs) into Beige Adipocytes

7:23

In Vitro AAV Infection of SVFs

8:11

Results: SPH-Induced Expression of Endogenous Prdm16 for Enhanced Oxygen Consumption

9:23

Conclusion

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