Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
2.2K Views
•
09:52 min
•
January 6th, 2023
DOI :
January 6th, 2023
•Chapters in this video
0:05
Introduction
0:53
Molecular Cloning
4:34
In Vivo Injection of Adeno-Associated Virus (AAV) into the Inguinal White Adipose Tissue (iWAT)
6:14
In Vitro Differentiation of Stromal Vascular Cells (SVFs) into Beige Adipocytes
7:23
In Vitro AAV Infection of SVFs
8:11
Results: SPH-Induced Expression of Endogenous Prdm16 for Enhanced Oxygen Consumption
9:23
Conclusion
Related Videos
07:03
Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
9.8K Views
11:11
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
2.2K Views
06:28
Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
12.8K Views
07:22
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
38.6K Views
11:24
Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments
1.7K Views
08:16
Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity
2.0K Views
09:00
Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
11.3K Views
08:31
Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
3.0K Views
04:46
Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
1.3K Views
03:58
Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System
2.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved