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Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

1.1K Views

09:02 min

September 22nd, 2023

DOI :

10.3791/64868-v

September 22nd, 2023


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Primary Nasal Epithelial Cells

Rozdziały w tym wideo

0:04

Introduction

0:57

Infection of Nasal Air‐Liquid Interface (ALI) Cultures

2:53

Collection and Quantification of the Shed Virus in Apical Surface Liquid (ASL)

3:49

Transepithelial Electrical Resistance (TEER) Measurement

5:25

Measuring Cytotoxicity by Lactate Dehydrogenase (LDH) Assay

6:18

Results: Characterizing Viral Replication, Host Cell Tropism, and Virus‐Induced Cytotoxicity in Nasal ALI Cultures

8:11

Conclusion

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