Ultrastructural Expansion Microscopy in Three In Vitro Life Cycle Stages of Trypanosoma cruzi
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09:45 min
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May 12th, 2023
DOI :
May 12th, 2023
•María Azul de Hernández*1, Gonzalo Martinez Peralta*1,2, Rodrigo Vena1,4, Victoria Lucia Alonso1,3,4
1Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 2Area Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 3Area Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 4Unidad de Microscopía, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET
Rozdziały w tym wideo
0:00
Introduction
1:16
Trypanosoma cruzi Culture
3:18
Cross-Linking Prevention and Gelation of T.cruzi Parasites
5:02
Denaturation of Gelified Samples and Isotropic Expansion of T.cruzi Parasites
6:28
Fluorescent Labeling and Imaging of T.cruzi Target Proteins
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