Ultrastructural Expansion Microscopy in Three In Vitro Life Cycle Stages of Trypanosoma cruzi

617 Views

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09:45 min

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May 12th, 2023

DOI :

10.3791/65381-v

May 12th, 2023

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María Azul de Hernández*¹, Gonzalo Martinez Peralta*¹^,², Rodrigo Vena¹^,⁴, Victoria Lucia Alonso¹^,³^,⁴

¹Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, ²Area Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), ³Area Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), ⁴Unidad de Microscopía, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET

Summary

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Ultrastructural Expansion Microscopy

Trypanosoma Cruzi

Chagas Disease

Super Resolution Imaging

Electron Microscopy

Nanoscale Structures

Cell Cycle Differentiation

High Resolution Imaging

Epimastigote Suspension

Chapters in this video

0:00

Introduction

1:16

Trypanosoma cruzi Culture

3:18

Cross-Linking Prevention and Gelation of T.cruzi Parasites

5:02

Denaturation of Gelified Samples and Isotropic Expansion of T.cruzi Parasites

6:28

Fluorescent Labeling and Imaging of T.cruzi Target Proteins

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