ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

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1.2K Views

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06:47 min

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August 25th, 2023

DOI :

10.3791/65828-v

August 25th, 2023

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Sarah Wazir¹, Danny Farhat¹, Mahalashmi Srinivasan¹^,², Jyh-Yeuan Lee¹

¹Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, ²Department of Biochemistry, Faculty of Medicine and Dentistry, University of Alberta

Podsumowanie

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ABCG5 G8

Crystallization

Lipid Bicelle

X ray Crystallography

ATP Binding Cassette Transporter

Detergent Solubilization

Rozdziały w tym wideo

0:00

Introduction

1:53

Reductive Methylation and Pre‐Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein

4:26

Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion

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