Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

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5.0K Views

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04:51 min

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December 29th, 2023

DOI :

10.3791/66197-v

December 29th, 2023

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Trinity Cookis¹, Paul Sauer²^,³, Simon Poepsel⁴^,⁵, Bong-Gyoon Han⁶, Dominik A. Herbst¹^,²^,⁶, Robert Glaeser⁶, Eva Nogales¹^,²^,³^,⁶

¹Department of Molecular and Cell Biology, University of California, Berkeley, ²California Institute for Quantitative Biology (QB3), University of California, Berkeley, ³Howard Hughes Medical Institute, University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD), University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division, Lawrence Berkeley National Laboratory

Podsumowanie

Przeglądaj więcej filmów

Cryo electron Microscopy

Sample Preparation

Affinity Grid Fabrication

Sample Denaturation

Preferential Orientations

Hydrophobic Interface

Protein Complexes

Two dimensional Crystals

Lipid Monolayer

Protocol Optimization

Troubleshooting Guide

Cryo EM Community

Rozdziały w tym wideo

0:00

Introduction

1:13

Designing Streptavidin Affinity Grids for Cryo‐Electron Microscopy

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