链霉亲和素亲和网格制备用于冷冻电子显微镜样品制备

Please note that all translations are automatically generated. Click here for the English version.

5.0K Views

•

04:51 min

•

December 29th, 2023

DOI :

10.3791/66197-v

December 29th, 2023

•

Trinity Cookis¹, Paul Sauer²^,³, Simon Poepsel⁴^,⁵, Bong-Gyoon Han⁶, Dominik A. Herbst¹^,²^,⁶, Robert Glaeser⁶, Eva Nogales¹^,²^,³^,⁶

¹Department of Molecular and Cell Biology, University of California, Berkeley, ²California Institute for Quantitative Biology (QB3), University of California, Berkeley, ³Howard Hughes Medical Institute, University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD), University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division, Lawrence Berkeley National Laboratory

副本

摘要

探索更多视频

JoVE 202

此视频中的章节

0:00

Introduction

1:13

Designing Streptavidin Affinity Grids for Cryo‐Electron Microscopy

相关视频

08:27

单粒子冷冻电子显微镜在结构中测定脂质敏感分通道 trpc3 的表达与纯化

9.4K Views

05:47

探头电喷电镀质谱的样品制备

9.3K Views

10:05

快速网格准备时间解决低温电子显微镜

3.9K Views

06:10

使用支撑浮块在透射电子显微镜中制备样品支撑膜

4.3K Views

09:06

冷冻电子断层扫描 的糖核 酸的准备和冷冻-FIB微加工

4.1K Views

11:52

单颗粒冷冻电子显微镜:从样品到结构

8.2K Views

05:05

冷冻电镜高温样品网格的制备

3.4K Views

09:53

微图案透射电子显微镜网格，用于在全细胞冷冻电子断层扫描工作流程中指导细胞定位

6.4K Views

04:22

使用脂质单层方法进行电子晶体学研究的样品制备

3.8K Views

13:43

强大的单颗粒冷冻电子显微镜（cryo-EM）处理工作流程，具有冷冻 SPARC、RELION 和 Scipion

12.6K Views