JoVE Logo

Zaloguj się

Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing

2.5K Views

05:58 min

August 16th, 2024

DOI :

10.3791/66766-v

August 16th, 2024


Przeglądaj więcej filmów

Dissociation

Rozdziały w tym wideo

0:00

Introduction

1:40

Rapid Dissociation of Tumor Tissue to Gain Enhanced Single‐Cell Tumor Insights

2:59

Red Blood Cell Lysis in Digested Tumor Samples for Single‐Cell RNA Sequencing

4:03

Dead Cell Removal from Dissociated Tumor for Single‐Cell Sequencing

Powiązane Filmy

article

10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

14.9K Views

article

06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

12.0K Views

article

06:44

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues

2.5K Views

article

09:14

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

711 Views

article

07:10

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

12.6K Views

article

09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

10.7K Views

article

05:06

Mechanical Dissociation of Tissues for Single Cell Analysis Using a Motorized Device

873 Views

article

10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

18.4K Views

article

04:03

Mouse Wound Models and Preparation of Single-Cell Suspensions

400 Views

article

07:12

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

3.9K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone