Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions
867 Views
•
07:16 min
•
January 5th, 2024
DOI :
January 5th, 2024
•Chapters in this video
0:00
Introduction
1:50
Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation
3:02
Preclearing of Transfected HEK293 Protein with the Protein G Gel and Capturing Protein Complexes Using Epitope Tag Affinity Gel
4:17
Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein‐Protein Interaction Studies
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