The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

0:15

Introduction

1:52

Step 1: Collect Embryos

2:24

Step 2: Prepare a Dechorionation Slide

2:51

Step 3: Prepare Imaging Chamber

3:32

Step 4: Place Halocarbon Oil on a Coverslip

4:05

Step 5: Picking Embryos From Plates

4:38

Step 6: Place Embryos onto Tape

5:01

Step 7: Roll Embryos to Remove the Chorion

6:15

Step 8: Transfer Embryos to Halocarbon Oil

6:47

Step 9: Push Embryos to Bottom of Oil

7:14

Step 10: Invert Coverslip on Live Imaging Chamber

7:38

Step 11: Secure Coverslip with Tape