Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

1:43

Generation of Stable Cell Lines Expressing 6His-Flag-Ubl

3:36

Double Purification of Modified Proteins

6:25

Processing of Mass Spectrometry Data to Generate Profiles of Ub/Ubls PTMs (Ubiquitin/Ubiquitin-likes Post-Translational Modifications)

7:45

Results: Ubiquitinated Proteins

9:13

Conclusion