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JoVE Journal

Bioengineering

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Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts

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07:13 min

June 20th, 2011

June 20th, 2011

21,755 Views

0:05

Title

1:43

Ovarian Dissection and Tissue Isolation

3:20

Degradation of Alginate and Collection of Cells

4:23

Fixation of Alginate Encapsulated Organ Cultures for Paraffin Sections

4:46

Application of the 3D Ovarian Organ Culture System

6:29

Conclusion

Culture of normal cells in their three-dimensional context represents an alternative method to study early events required for cellular transformation and tumorigenesis. This method is used to grow normal ovarian and oviductal cells to study early events in ovarian cancer formation.

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