August 20th, 2012
•We demonstrate FRET between conjugated polymer polydiacetylene (PDA) and fluorophore attached to the surface of PDA liposomes for the sensing of biomolecules. PDA liposomes also contained receptor molecules on their surfaces for biomolecules to be used as probes. Ligand-receptor interactions lead to changes in the FRET efficiency between the fluorophore and PDA which is the basis of the sensing mechanism.
Tags
Vídeos Relacionados
Time-lapse Imaging of Mitosis After siRNA Transfection
Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados