Lentiviral Transduction and Stable Line Generation
4:53
Dose Response Effect of Proteosome Inhibitors on Protein Aggregation and Image Analysis
7:06
Time lapse Imaging of YFP Expression in Living Cells
8:09
Results: Quantification of SOD1 Mutant Protein Aggregate Formation
10:04
Conclusion
Transcrição
The overall goal of this experiment is to develop an assay for studying the effect of small molecules in modulating the aggregation of misfolded proteins in a time and dose dependent manner. This method can help answer the key questions for develo
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We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.