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Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

28.6K Views

08:13 min

October 17th, 2017

October 17th, 2017

28,563 Views

0:05

Title

0:41

Sterilization of Arabidopsis Seeds with Bleach

2:14

Sterilization of Arabidopsis Seed with Chlorine Gas

5:01

Seed Plating and Evaluating Germination

5:59

Results: Effects of Bleach or Chlorine Gas Sterilization on Seed Germination

7:28

Conclusion

Transcrição

The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.

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