October 24th, 2018
•This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.
Vídeos Relacionados
Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados