Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
7:25
Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells
9:31
Conclusion
Transcript
This protocol introduced a defined feeder cell free method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells. Limbal epithelial stem cells are responsible for renewing the corneal epithelial in the healthy
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This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.