Extraction of Circulating miRNAs from Plasma Samples
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Perform Reverse Transcription and miRNA Pre-amplification
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Perform Real-time PCR
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Results: Increase in hsa-miR-155-5p Associated with Shorter PFS and OS
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Conclusion
Transcrição
This method can help answer key questions in the translational research field, such as the relationship between circulating biomarkers and the patient prognosis. The main advantage of this technique is the chance to evaluate the expression of a pa
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We present a protocol to evaluate the expression levels of circulating microRNA (miRNAs) in plasma samples from cancer patients. In particular, we used a commercially available kit for circulating miRNA extraction and reverse transcription. Finally, we analyzed a panel of 24 selected miRNAs using real-time pre-spotted probe custom plates.