January 25th, 2019
•Dictyostelium discoideum is a popular model organism to study complex cellular processes such as cell migration, endocytosis, and development. The utility of the organism is dependent on the feasibility of genetic manipulation. Here, we present methods to transfect Dictyostelium discoideum cells that overcome existing limitations of culturing cells in liquid media.
Vídeos Relacionados
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
Single Molecule Analysis of Laser Localized Psoralen Adducts
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados