November 1st, 2019
•Here, we present a protocol to enrich, isolate, identify, and characterize proteins modified by SUMO in vivo both from human hepatoma cells and liver tumors obtained from mouse models of hepatocellular carcinoma by using SUMO-binding entities (SUBEs).
Vídeos Relacionados
Identification of Fatty Acids in Bacillus cereus
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Isolation of Tissue Extracellular Vesicles from the Liver
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados