RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

1:27

Step 1: Preparation of a 15% Denaturing Polyacrylamide Gel

2:03

Step 2: Assembly of the Glass Plates and Electrophresis Unit

4:13

Step 3: Preparation of Loading Dye and Samples

5:38

Step 4: Gel Electrophresis

6:33

Step 5: Preparation for Electro-blotting

10:15

Step 6: Preparation of Radiolabeled Probe

10:50

Step 7: Hybridization of the Blot

12:36

Representative Results

13:24

Conclusion